Labeling the cells in a sample is one of the most important steps in any lab work. In this article, the writer discusses how real-time PCR system can help automate certain tasks previously done by human staff.
What is real-time PCR?
Real-time PCR is a technique that allows scientists to quickly and accurately identify the location of specific DNA sequences in a sample. This technology can be used to study gene expression and detect genetic mutations.
How does real-time PCR work?
The real time PCR system uses a light-emitting diode (LED) as the probe, which captures fluorescent signals emitted by the nucleic acid molecules. The LED is constantly monitored, and when it detects a fluorescent signal corresponding to the target DNA sequence, it triggers an electronic circuit that records the amount of amplification. This allows researchers to track the progression of the reaction at real time without having to wait for a cycle completion marker like standard PCR.
Applications of the Real-Time PCR System
Applications of the Real-Time PCR System can be found in a variety of fields. For example, the system can be used to detect viruses and bacteria in biological samples. Additionally, the system can be used to determine the sequence of DNA, including the detection of COVID-19.
The real-time PCR system is a powerful tool that lab professionals use to determine the presence of specific DNA sequences in a sample. By using this technology, scientists can confirm the identity of any type of DNA sequence and track its progress through the laboratory process. If you are working with DNA samples in your laboratory, it is important to have a real-time PCR system at your disposal so you can make accurate determinations and keep track of your experiments as they proceed.